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The Ku70/80 heterodimer binds to DNA ends with high affinity and specificity. The protein forms a ring that can thread onto a DNA end, which explains its inability to bind to circular DNA molecules or internal positions on the chromosome . Once bound to DNA, Ku70/80 attracts the catalytic subunit of the DNA-dependent protein kinase (DNA-PK CS).

et al. (2012) explored the role of Ku70/80 more closely and found that the C-terminal region of the Ku80 subunit is the main structural element that forms the interactions important to the function of Ku70/80 in bringing together the broken DNA ends. The data from Bennett et al. is consistent with the crystal structure as determined by Walker 2015-01-01 KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation Simplified, Ku70/80 complex is a sensor of DSBs that initiates the assembly of C-NHEJ complex to restore integrity of a DNA molecule. In fact, the role of Ku70/80 proteins is highly complex and integrates different aspects of DNA repair. 2012-02-10 2013-07-01 2004-07-09 KU70 – Kontrolluppgift – Näringsbidrag och royalty (SKV 2316) På skatteverket.se använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig.

Ku70 80 function

Here we report that KU70 and DNA-PKcs unexpectedly function together during the induction of apoptosis after exposure to high levels of etoposide. In the presence of 100 μM etoposide, apoptosis was induced within 1 h in wild type DT40 cells but not in KU70-/-and DNA-PKcs-/-/-cells. In addition, the DNA-PK inhibitors NU7026 and wortmannin, as well as the caspase inhibitor Z-VAD-FMK, inhibited etoposide-induced apoptosis in wild type cells. Ku70/80 also interacts with the telomeric factor Trf1 and plays an important role in telomere maintenance (Bailey et al., 1999; Hsu et al., 1999, 2000; Gasser, 2000). Conformational changes in Ku70/80 could affect this interaction and impinge upon whether Ku70/80 binds Trf1 and acts at telomeres, or alternatively participates in NHEJ. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications.

Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat, Xenopus laevis samples. Supplied as 500 µL purified antibody (0.2 mg/mL).

Required for mating-type switching (By similarity). 1999-01-01 Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications.

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2. Results 2.1.

av P Håkansson · 2006 — regarding the function of an alternative mammalian RNR small subunit, and on the role of is the binding of the end-binding Ku70/Ku80 complex, leading to the. av J Alfredsson Timmins · 2009 — forms of the HP1 proteins that perform slightly different functions (Lomberk et al., 2006 choring of the telomeres to the NM is facilitated by Ku70, Esc1, members of the Sir protein Hum Genet 80, 235-46. Cremer, T. and Protection of Kidney Function with Human Antioxidation Protein Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in By agreement with the company, the product's function will not be exposed in the report. of CUX1 with some of these proteins: REV1, PARP1 and Ku70/Ku80. (Abstract); Kuci V, Nordström L, Jerkeman M, Ek S (2015) Emerging role of of 111In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer.
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av J Fransson · 2006 — Functionally-associated target antigens in cancer. id: 5decc108-1738-478b-80d5-d92c6f2f70a5 (old id 547281); date added to LUP: 2016-04-04 12:19:11 is feasible, with CDK12 absence possibly signifying defective DDR function. reduced expression of a number of DDR proteins including ATR, Ku70/Ku80, PCR förstärka en kb 5 'uppströms sekvens av Ku70 genen med glycerol till en slutlig koncentration av 25% (v / v) och förvara vid -80 ° C för långtidsförvaring. Analysis of regions essential for the function of chromosomal exempelvis Rad51, DNA-PK, Ku70/80, DNA-ligas IV, BRCA1 och BRCA2.

Once bound to DNA, Ku70/80 attracts the catalytic subunit of the DNA-dependent protein kinase (DNA-PK CS). Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection.
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The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA. Databases. EM maps have been deposited in EMDB (EMD‐11933). Coordinates have been deposited in Protein Data Bank

Conformational changes in Ku70/80 could affect this interaction and impinge upon whether Ku70/80 binds Trf1 and acts at telomeres, or alternatively participates in NHEJ. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat, Xenopus laevis samples. Supplied as 500 µL purified antibody (0.2 mg/mL). Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V (D)J recombination product formation defects.